FOOD. Science. Technology. Quality

Food. SCIENCE. Technology. Quality

Food. Science. TECHNOLOGY. Quality

Food. Science. Technology. QUALITY

Authors

AGATA GÓRSKA, KAROLINA SZULC, EWA OSTROWSKA-LIGĘZA, MAGDALENA WIRKOWSKA, JOANNA BRYŚ

Title

Attempt to use β-lactoglobulin as carrier for retinyl palmitate in non-fat systems

Abstract

β-lactoglobulin has a capacity to bind hydrophobic ligands owing to the presence of β-barrel in its structure, and this capacity can be utilized to create compounds with retinyl palmitate. The scope of this research study comprised the synthesis of complexes of β-lactoglobulin and retinyl palmitate in phosphate buffer solutions of various pH values. The products obtained were spray and freeze dried in order to produce powders thereof due to the universality of their applications functionality in food industry. In the samples produced, there were determined the content of retinyl palmitate by a HPLC method and the physical parameters, i.e: water content, water activity, size of particles, solubility, and wettability as the parameters critical to the quality of final products. The research confirmed the possibility of forming complexes between β-lactoglobulin and retinyl palmitate. It was proved that the temperature of drying process and the pH value had a significant impact on the binding capacity of the β-barrel. The drying method had a significant impact on the particle size of the preparations in the form of powders. The complex obtained by freeze drying was characterized by the largest size of the particles. The studied complexes of β-lactoglobulin and retinyl palmitate had a very good solubility regardless of the drying method and process parameters.

Keywords

β-lactoglobulin, retinyl palmitate, binding properties, carrier

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