The aim of this study was to analyze staling of wheat and mixed bread using acoustic method, and to recognize relationship between acoustic properties and the time of storage of both breads. Breads were. stored at 25°C and RH 25-30%. Acoustic emission was measured during puncture test using piezoelectric accelerometer between 1-15 kHz frequency. Puncture test was done in Zwick Machine 1445 with velocity of 1 mm/s and to the depth of 20 mm. Moisture content in crust and crumb was measured with standard drying method. Structure of bread was analyzed with scanning microscopy. Water content in crust and crumb of both breads decreased during storage, and the decrease was more pronounced in wheat bread. Acoustic emission increased during storage and was dependent on the kind of analyzed bread. Wheat bread emitted acoustic signal with frequency from 2 to 6 kHz, while mixed bread emitted acoustic signal in two bands, from 2 to 5 kHz and from 13 to 14 kHz. Both analyzed breads differed substantially in microscopic structure. It is concluded that acoustic emission can be used to assay staling of bread.