The research results come from a field experiment which was carried out over 2002-2004. The objective of the paper was to determine the effect of the soil tillage systems (traditional and simplified) and weed control methods for herbicides (Plateen 41,5 WG, Plateen 41,5 WG + Fusialde Forte 150 EC, Plateen 41,5 WG + Fusialde Forte 150 EC + adjuvant Atpolan 80 EC, Barox 460 SL, Barox 460 SL + Fusialde Forte 150 EC, Barox 460 SL + Fusialde Forte 150 EC + adjuvant Atpolan 80 EC) on the content of ascorbic acid in the edible potato tubers cv. Wiking. The obtained results showed that the level of ascorbic acid depended significantly only on the weather conditions during the vegetation periods. During hot and dry vegetation weather the potato tubers accumulated most ascorbic acid. It was stated that the content of ascorbic acid decreased after preliminary processing (after peeling) mean for 15,3 mgּ·kg^-1 fresh matter as compared to level of acid non-peeled potato tubers.