The objective of the research study was to determine the effect of pasteurization (no pasteurization; pasteurization at 72°C/15 s, 85°C/15 s, and 95°C/10 min) and homogenization of milk (no homogenization; homogenization at 6 MPa ) on the content of cholesterol in milk and in rennet bundz- type cheeses made from that milk. It was found that those two processes impacted, statistically significantly, the cholesterol content in both the milk and the cheeses. Pasteurization caused the content of cholesterol in milk to increase. However, its level decreased in the cheeses and the decrease was proportional to the increase in the pasteurization temperature. The content of cholesterol in raw milk and in the cheeses produced from it amounted to 14.41 mg/100g and 115.61 mg/100g, respectively; after they were pasteurized at 95°C/10 min, it amounted to 19.34 mg/100 g and 55.01 mg/100g, respectively. The homogenization process of raw milk caused the cholesterol levels to decrease in both the milk and the cheeses to a level of 5.55 mg/100 g and 88.48 mg/100g, respectively. Based on the analysis of the simultaneous effect of the two factors, it was proved that, along with the increasing pasteurization temperature, the level of cholesterol in cheeses decreased to a greater degree in those products that were made from homogenized milk.