The objective of the investigations was to determine the effect of content level of retrograded, acetylated starch (RS4 resistant starch) in dough and bread on the quality properties of dough and wheat bread. In the experiment, the following content levels of resistant starch were applied: 0, 10, 20, 30 and 40 %. To balance the loss of wheat gluten resulting from replacing 10-40 % of the flour by resistant starch, 1.5% of vital wheat gluten was added to the mixtures with the content of resistant starch being 10 and 20%, and 3% to the mixtures with the content of the retrograded, acetylated starch being 30 and 40%. In the investigations, the following was determined: quality characteristics and amylographic features of wheat flour enriched with resistant starch, and farinographic (rheological) properties of the dough. Next, bread was baked and its quality was evaluated. The investigations proved that the content level of resistant starch of up to 10% did not significantly reduce the bread quality. It was found that the increasing content of resistant starch caused the values of quality parameters, initial temperature of gelatinization, and of bread volume to decrease. The final temperature and the gelatinization time increased along with the rising amount, up to 30%, of the resistant starch added, and in the samples containing 40% of resistant starch, those two parameters showed the lowest values. The increasing content of RS4 starch positively impacted the water absorption of flour, development time of dough, quality number, and overbake of bread. The vital gluten added had an effect of increasing the content of total protein, sedimentation value, wet gluten yield, water absorption of flour, and bread volume.