Lipids can undergo autooxidation leading to undesirable compounds formation. In an effort to retard the processes involved, various natural antioxidants are applied. In our paper, the antioxidant activity of the ethanol extract from wheat bran on the oxidative stability of rapeseed oil was studied. The extract was added at the 0.1 and 0.3% vol. concentration. The results of the peroxide and thiobarbituric acid values have shown that the wheat bran extract has an antioxidant effect. The inhibition effect in percentage was 34.4%, and 46%respectively for 0.1%, and 0.3% addition of wheat bran ethanol extract. Total phenolics in wheat bran extract (0.08 mg TAE/g dry matter) were determined spectrometrically using Folin-Ciocalteu reagent and expressed as tannic acid equivalents (TAE).