The aim of the study was to obtain haploid populations from brewer’s yeasts. Two brewing strains: R-II and R-9 were examined. Only strain R-9 was able to sporulate. Spore viability was 14%. 20 monospore populations were obtained, 16 of them were haploid (possessed mating type). One population (R-9/12) was able to sporulate, asci were dissected. Spore viability was 30%. 13 monospore populations were obtained, 10 of them were haploid.