The objective of this research was to assess the quality of wheat-barley dough and bread with and without the vital gluten added, and made with barley sour doughs fermented by starter cultures. In a series of laboratory bakings, doughs containing 30, 40, and 50% of barley flour with, respectively, 5 or 10% of the vital gluten added, were manufactured with barley sour doughs fermented using freeze-dried LV1 and LV2 starter cultures. It was found that the amount of whole barley flour introduced into the dough in a form leavened by LV1 or LV2 starters caused the dough yield and phase acidity to increase, and the fermentation time of wheat-barley dough pieces to decrease. The percent content of the whole barley flour leavened by starter cultures in the dough bulk and the type of starters caused the quality properties of the baked products to vary. Breads containing 30-50% of the whole barley flour and produced using barley sour doughs made with an LV2 starter culture were characterized by a greater volume, good crumb elasticity, lower acidity, and a higher sensory quality in comparison with the breads manufactured using sour doughs fermented by an LV1 starter. The addition of 5-10% of vital gluten to the wheat-barley dough manufactured with sour doughs fermented by LV1 and LV2 starters improved the quality of both the dough and the breads. The increased levels of the gluten added to the dough bulk caused the dough yield to increase, the dough acidity to decrease, the bread volume to rise, and the sensory characteristics of breads to improve if compared with the samples without the addition of vital gluten. It was found that the LV2 cultures could be utilized as fermentation starters to produce nutritionally valuable wheat-barley breads. However, in order to obtain good quality breads containing 50% of the whole barley flour, it is necessary to add 10% of the vital gluten.