In the reference literature available, there are reports that the limited availability of nutrients in the culture medium of micro-organisms induces their resistance against antimicrobial factors. The objective of the conducted experiments was to verify this research hypothesis. The effect was determined of the limited availability of nutrients in a culture medium on the resistance of Enterococcus faecalis, Proteus vulgaris, and Pseudomonas aeruginosa against the action of three selected disinfection agents: formaldehyde, hydrogen peroxide, and quaternary ammonium salts. A suspension method was applied to determine MIC_90% (minimal inhibitory concentration), i.e. minimal concentration levels inhibiting the growth of micro-organisms in 90% disinfection agents studied, and MBC (minimal bactericidal concentration), i.e. minimal, bactericidal concentration levels. The analysis of changes in the profile of total proteins of micro organisms was performed using a single-direction electrophoresis method under denaturing conditions (known as SDS-PAGE method, i.e. sodium dodecyl sulfate polyacrylamide gel electrophoresis). Based on the experiments accomplished it was found that neither the limited availability of nutrients in the culture medium nor the time of incubation had any impact on the increase in the resistance of microorganisms treated by the three selected disinfectants. The quaternary ammonium salts proved to have the most effective bactericidal activity against Enterococcus faecalis, whereas in the case of Proteus vulgaris and Pseudomonas aeruginosa cells – the formaldehyde agent. It was also found that the incubation of Pseudomonas aeruginosa cells grown in a medium with a limited availability of nutrients induced changes in the profile of general proteins of this micro-organism.