Efficiency of enzyme action on starch was tested. The following enzymatic preparations were used: AMG 300L and AMG E -glucoamylase, Dextrozyme 225/75L and Dextrozyme E -combination of glucoamylase and pullulanase, Promozyme 200L – pullulanase. Maltodextrine, wheat and tapioca starches were used as a substrate. The tested enzymatic preparations were applied for saccharification individually or in combination. Research was carried out at fixed parameters: temperature, pH, starch concentration and enzyme dosage. The research indicated that the enzymatic preparations obtained from genetically modified microorganisms characterised higher efficiency. Application in similar dosage of Dextrozyme E instead of AMG 300 makes possible to obtain hydrolysates with higher DE by 16-32% or to produce hydrolysates with similar DE within 24 h.