Falcarinol and falcarindiol are two major polyacetylenes in Apiaceae family. These compounds were placed on the list of Natural Product with Antitumor Activity created by National Cancer Institute, USA. The largest amount of polyacetylenes occurs in plant roots. Celeriac as a root vegetable is not known very well worldwide. In many countries only leaf varieties (celery) are valued, therefore there are few studies on celeriac polyacetylenes. The purpose of this research was to analyze varieties of celeriac (Apium graveolens L. var. rapaceum) available in Poland in terms of polyacetylenes. The research was conducted on 18 varietes of celeriac: ‘Jabłkowy’, ‘Odrzański’, ‘Makar’, ‘Brilliant’, ‘Diamant’, ‘Gol’, ‘Luna’, ‘Mentor’, ‘Monarch’, ‘Cascade’, ‘Feniks’, ‘Edward’, ‘President’, ‘Prinz’, ‘Snehvide’, ‘Cisko’, ‘Dukat’ and ‘Talar’. Gas chromatography mass spectrometry method (GC/MS) was employed to analyze the content of polyacetylenes. Two polyacetylenes – falcarinol and falcarindiol were identified. The largest amount of falcarinol was found in ‘Makar’ and ‘Jabłkowy’ celeriac variety and it was measured to be 25.34 and 22.37 mg·100 g^-1 of dry mass respectively. ‘Odrzański’ and ‘Luna’ varieties contained the highest amount of falcarindiol which was measured to be 10.13 and 12.55 mg·100 g^-1of dry mass.