The objective of this study was to investigate changes in the physical and chemical properties of milkfat + rapeseed oil mixtures after the interesterification process performed. The fats interesterification process was carried out 2 and 8 hours, at a temperature of 60°C, in the presence of an enzymatic preparation ‘Novozym 435’. The chemical interestericifation reactions were also performed in the presence of sodium methoxide used as a catalyst, at a temperature of 60°C; the reaction durations were 0.5 and 1.5 hours. The following parameters were determined in the mixtures prior to and after the interesterification process accomplished: fat acid values, polar fraction content, slip melting points, solid fat content, and fatty acids compositions. It was stated that the intersterification process accomplished resulted in the increase in both the free fat acids content and polar fraction content, and this increase was higher in chemically interesterified mixtures. Products of the chemical interesterification process of a mixture containing 25% milkfat showed lower slip melting temperatures and contained a solid fat fraction while products of the mixture interesterification process with the 75% milkfat content had a higher melting temperature with regard to physical mixtures. As expected, the distribution of fatty acids among the sn-2 and sn-1,3 positions of triacylglycerols of interesterified fats appeared to be close to the statistical distribution. By selecting and adjusting the conditions under which any interstrification process is conducted, it is possible to obtain final process products that are various fats of a variety of technological applications.