The objective of the research study was to determine the effect of enzymatic interesterification on the quality of fat used to bake cookies for young children. A mixture of milkfat, rapeseed oil, and fish oil concentrate (4 : 5 : 1) was enzymatically interesterified with the use of a Lipozyme RM IM preparation. The cookies were baked on the basis of the interesterified fat. In the interesterified mixture and in the fat extracted from the cookies, fatty acids were found, which originated from milkfat and from the long-chain polyunsaturated fatty acids: eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Their amounts in the interesterified mixture were, respectively: EPA – 1.48 % and DHA – 1.66 % DHA; their amounts in the fat from cookies were, respectively: EPA – 1.29 % and DHA – 1.42 %. The interesterification products produced and the fat extracted from the cookies were characterized by the fatty acid composition and structure of triacyglycerols similar to that in human milkfat. More than 60% of palmitic acid was esterified in the internal position of triacyglicerols, whereas the unsaturated fatty acids – in the external positions.