The objective of this research was to estimate the effect of beef grinding on the accuracy of estimating fat content using a computer-aided video image analysis. Photographs of the analyzed beef trimmings from the fabrication line were taken. Next, each meat sample was ground in a grinder with a plate having 10 mm openings and, again, photographs of the meat ground were taken. Based on the photographs taken, a computer-aided video image analysis was performed. Furthermore, in the meat analyzed, a reference fat content was determined using a standard Soxhlet method. The parameters as determined by the VIA method (i.e. the RGB colour values and the content of white fields – corresponding with the fat and connective tissue) were correlated with fat content determined by a reference method. The results obtained were statistically analyzed and this analysis disclosed significant dependencies between the content of white fields determined by the VIA method and the fat content determined by the Soxhlet method in the case of both the non-ground and the ground meat. The similar values of correlation coefficients referring to the non ground and ground meat allow for the statement that the grinding degree has not a big effect on the accuracy of estimating the fat content therein using the computer-aided video analysis method.