Specific sugar profiles of six different origins nectar honeys were established. Separation of honey sugars by HPLC resulted in following fractions: fructose, glucose, di-, trisaccharides and two fractions of higher oligosaccharides; first one containing tetra-, penta and hexasaccharides and a second oligosaccharides higher than heptasaccharides. The contents of particular fractions in a dry mass of honeys were determined as well as ratios of fructose to glucose concentrations (F/G). Some correlations between a sugar composition and a type of honey were found, however, only rape honeys presented really distinctive relation. These honeys differed significantly from other types, i.e. contrary to others rape honeys contained more glucose than fructose, the lowest concentration of di- and trisaccharides and did not contain or contained very low amounts of tetra-, penta- and hexasaccharides. Acacia honeys featured a highest concentration of fructose, di- and trisaccharides but the lowest concentration of glucose and oligosaccharides higher than heptasaccharides. These sugars were found in the highest concentration in linden honeys. It was also found that the concentration of oligosacharides higher than heptasacharides in rape, linden, floral and heather honeys was higher than trisaccharides,