The objective of the research study was to determine the effect of industrial extraction and refining of rapeseed oil on the profile and content of chlorophyll pigments, where those processes were carried out under industrial conditions with the use of a modernized production line. The material to be analysed were samples of oil collected at various stages of the rapeseed oil extraction and refining processes. The content of chlorophyll pigments was determined using a reversed phase high-performance liquid chromatography with fluorometric detection. It was found that the industrial method of extracting rapeseed oil affected the profile and content of chlorophyll pigments. The content of chlorophyll pigments in the extracted oil was 40 % higher than that in the pressed oil. The chlorophyll pigments analysed mainly contained pheophytin and phyropheophytin, and only small amounts of chlorophylls a and b. The thermal treatment of oil performed prior to the refining caused the chlorophylls to fully transform. The phyropheophytin to pheophytin ratio changed from 0.7 to 3.8 : 1. The crude oil contained chlorophylls derivatives only. The aciddegumming and neutralisation processes of oil caused the total content of chlorophyll pigments to slightly decrease and their profile to change a little. The effectiveness of reducing chlorophyll pigments after bleaching was 98.5 %. Compared to the chlorophyll b derivatives, the chlorophyll a derivatives were more easily removed from the oil with the use of bleaching clay. In some batches of the bleached oil the phyropheophytin b was still present and its amount was of 0.09 mg/kg.