The objective of this study was to compare the quality of meat from different cuts obtained from carcasses of roe deer (Capreolus capreolus L.) bucks hunted in forests in north-east (10 animals) and southeast Poland (10 animals). Four primal carcass cuts of bucks were analyzed: neck, shoulder, saddle, and leg. Meat obtained from each cut was finely chopped and thoroughly mixed. Next, average samples were collected from each cut (of ca. 300 g). The chemical composition and physicochemical properties of meat were determined. It was found that the meat of roe deer bucks from north-east Poland was characterized by a higher (p ≤ 0.01) content of dry matter, total protein and mineral compounds determined as ash, and a higher (p ≤ 0.05) average pH value. Furthermore, their meat was characterized by a lighter colour (L*) (p ≤ 0.01), it was also more yellow (b*) (p ≤ 0.01) and less red (a*) (p ≤ 0.01). The meat from shoulder had the lowest (p ≤ 0.01) content of dry matter. The meat from saddle had the highest content of protein, and the meat from shoulder: the lowest (differences among the means in the experimental groups were confimed statistically ). The meat from neck was characterized by the highest content of fat, the lowest content of mineral compounds determined as ash, and, also, the highest pH value (differences among the means in the experimental groups were confirmed statistically ). The colour of the meat from neck was lighter (L*) (p ≤ 0.01) compared with the colour of the meat from saddle and leg, its red component was the strongest (p ≤ 0.01) (a*) and its yellow component (b*) was also stronger (p ≤ 0.05) compared with the colour of the meat from leg. The meat from leg and saddle was less red compared with the meat from shoulder (p ≤ 0.01). Moreover, it was found that the meat from leg was less yellow (b*) (p ≤ 0.05) compared with the meat from shoulder.