The potential of reducing mycotoxin concentrations in maize grain was assessed during the process of producing ethanol with the application of classic technology (Henze’s steamer) and PLS technology (Pressureless Starch Liberation). The effect of individual operations, processes, and Saccharomyces cerevisiae yeast on changes in the concentrations of mycotoxins in mashes and slops was analyzed. As for the PSL technology, the dependence was found between the reduction of mycotoxin concentrations and the method of how the mash was thermally processed. Owing to “jet-cooker” heaters used, the reduction degree of mycotoxin concentration was higher and amounted to, at the maximum, ca. 20 % in the case of deoxynivalenol (DON), and up to 21 % in the case of fumonisins (FUM). Alternatively, when plate heat exchangers were utilized (elimination of contact between water steam and mash), the elimination of mycotoxins was less effective. In the classic technology, a considerable reduction of concentrations of DON, zearalenone (ZEA), and FUM was found when a barothermal processing was applied. The final balance of mycotoxin concentration in this technology showed that FUM were eliminated almost completely, DON at a level between 77 – 97 %, and ZEA was reduced by about 95 – 100 %. During the fermentation process, no impact of this process with the yeast used was found on the reduction of DON, ZEA, and FUM concentrations. The results obtained when the PLS technology was applied prove that no complete elimination of mycotoxins should be expected. Under specific conditions, the ZEA concentration in dried slops was found to be three times as high as in the raw material. Therefore, it is highly advised to control the slops produced during the processing of raw materials contaminated by mycotoxins, especially when they are used in feeding animals.