The objective was to study the effect of training and vitamin C supplementation on hepatic Ca, Mg, Fe, Zn, Mn and Cu concentration in rats. Animals were randomly divided into four groups fed ad libitum for 90 days with semipurified diets containing 1.47 MJ brutto energy per 100 g (350 kcal/100 g) and 20% of energy originated from protein. Two groups of rats were offered above diets enriched with vitamin C (375 mg/kg diet in total). It was the 15 fold higher concentration than in groups without supplementation. Rats of two groups, one fed without addition of vitamin C and the other supplemented with vitamin C, were trained for one hour daily. In trained rats higher liver concentration of minerals was observed, except iron. The significantly higher concentration was stated in case of: Mg (288 ±12 i 329 ±13 mg/kg), Zn (48.8 ±1.8 i 5.7.9 ±1.9 mg/kg) and Cu (4.50 ±0.18 i 5.36 ±0.19 mg/kg). Liver Cu concentration in untrained animals supplemented with vitamin C was also significantly higher (5.38 ±0.27 mg/kg) than in untrained group fed diet with normal vitamin C content (3.63 ±0.24 mg/kg).