The aim of this work was to find out to what degree different defatting conditions viz, solvents and temperature of extraction affect defatting efficiency and physico-chemical defatted starch properties. Triticale starch subjected to 10 h extraction using 75 % n-propanol at 30, 50 and 80°C, as well as mixture of chloroform:methanol:water (in volume proportion 18:6:1) at 30 and 50°C was used for investigation. Our studies confirmed the highest effectiveness of starch defatting with 75 % n-propanol at 80°C.