Corn oil is a rich source of bioactive compounds, including carotenoids and of unsaturated fatty acids, such as lipid alcohols, which include phytosterols and tocopherols. The corn oil production method determines the content of the above-mentioned compounds, and their amount depends on the initial preparation of plant material to extract oil. Corn grain contain 3.1 ÷ 5.7 % of oil, the predominant amount of which is found in the seed embryo. Corn oil for food use is produced from seed embryos. Applied are such oil extracting methods as pressing and solvent  extraction. Also, there are processes to produce oil from the whole corn seeds and corn bran, the properties of  which vary because of the different levels of bioactive substances contained therein. Corn seeds are processed not  only into edible oil, but also into bioethanol used as an additive to high-octane fuels. The production technology of  bioethanol makes it possible to obtain crude post-fermentation corn oil, which is a by-product characterised by a  higher content of phytosterols (0.6 ÷ 0.93 % w/w) and carotenoids (29.55 ÷ 40.53 mg/100 g). Until now, the post- fermentation crude oil is not used for food purposes, but only as a raw material for the production of biodiesel and  as a feed additive. Its pro-health values are disregarded. Corn oil is characterised by varied composition and it  is a potential source of biologically active substances.