The effect was examined of catechins, BHT, δ-tocopherol, and potassium sorbate on lipid emulsification in gastric and duodenal media, simulated in vitro. The experimental material was cottage butter; and the substances examined were added to the butter in the amount of 50 mg per 100 g of butter. The samples were emulsified for 2 hours, then, the percentage rate of emulsified lipids was measured as was the size of droplets in the emulsion produced. The results obtained were statistically analyzed and, based on this analysis, it was found that in the doses examined, BHT, δ-tocopherol, potassium sorbate, and catechins significantly limited the formation of emulsion and the degree of it dispersion. The power of their antiemulgative effect depended, however, on the process conditions applied. In the acidic gastric medium, δ-tocpoherol inhibited the emulsification to the highest degree. It reduced the amount of the emulsion formed to a level of only 7.5 % against 25 % found in the control sample, and increased the mean size of lipid droplets by 6.25 μm compared to the control sample. At this stage of the investigations, only catechins were a substance that was neutral towards the butter emulsification. An opposite situation took place in the alkaline duodenal medium. Under those conditions, the catechins and BHT inhibited the butter emulsification process to the highest degree, whereas the δ-tocopherol to the lowest degree. The presence of both the catechins and the BHT resulted in the decrease in the amount of emulsion formed from 83 % (control sample) to 62.5 %. The content of tocopherol in butter did not impact the amount of emulsion formed in the duodenal medium, but it significantly increased (by 11.14 μm) the mean size of lipid droplets forming this emulsion. The results obtained prove that the power of anti-emulgative effect of catechins in a dose guaranteeing their full antioxidant efficiency cannot be a factor excluding the possibility of using those substances as stabilizers of high-lipid products.
catechins, BHT, δ-tocopherol, potassium sorbate, lipids emulsification