A qualitative assessment was performed of two methods used to detect the addition of common flour and spelt flour in pasta made from durum wheat: electrophoretic separation of gliadin fraction of proteins and immunoenzymatic test of pasta quality control (PQC). Also, analyzed were some selected quality characteristics of grain of new durum wheat (STH 716, STH 717) and spelt (STH 3, STH 715) lines; the grain was used to make flour for pasta. Of the assessed lines, the STH 716 durum wheat flour was characterized by the best quality parameters, i.e. by a high protein content and an optimal falling number, whereas the caryopses by the highest vitreousness. Compared to the grain of the other wheat lines, the polyphenol oxydase activity of grain of the STH 716 and STH 717 durum wheat lines was three times lower. When determining the content of spelt wheat flour added, the PQC test results performed were encumbered with a bigger error than when determining the content of common wheat added. Based on the differences and similarities on electropherograms characteristic for the two wheat species, it is possible, using the electrophoretic separation of gliadin proteins extracted from the pasta, to distinguish between the common wheat and spelt wheat admixtures.
durum wheat, spelt, pasta adulteration, ELISA, electrophoretic distribution