FOOD. Science. Technology. Quality

Food. SCIENCE. Technology. Quality

Food. Science. TECHNOLOGY. Quality

Food. Science. Technology. QUALITY




Application of HPLC method for analysis of Se(IV) and Se(VI) in mineral waters


The aim of the study was application of a high performance liquid chromatography (HPLC) with UV detector for selenium speciation in samples of mineral water. Basic solutions 1000μg/l SeO32- and SeO42- were prepared by dissolving 2,190 g of Na2SeO3 and 2,393 g Na2SeO4 in 1000 ml of water, respectively. Recent standards SeO32- and SeO42- (single or mixed) were prepared every day from the basic separation of Se(IV) and Se(VI) was obtained using a C18 column (250×4.0mm; 5μm) with mobile phase comprising 5mmol/lphosphate tetrabutylammonium, 50/50 water.methanol at pH 3,4. Flow rate was 1ml/min. Detector was set at 210 nm. Injection volume was 5 μl. Retention time of selenium(IV) was 2.27 min and of selenium(VI) 4.37 min. Recovery of Se determined using a HPLC method was 99.6 %. The determined content of Se(IV) was 4,10 μg/l and Se(VI) – 4,27 μg/l. The method worked out can be therefore recommended for determination of Se in samples of mineral water.