The objective of the research experiment was to determine the distribution of ellagic acid and ellagitannins in the main material streams during the strawberry fruit juice-making process, with the application of a pectinolitic preparation. The laboratory-scale experiments were performed using fruits of a “Honeoye” strawberry cultivar counted among the best commercial strawberry cultivars from the point of view of the integrated and organic cultivation. The ellagic acid and sanguiin H-6 were determined using an HPLC method. It was proved that during the laboratory scale process of strawberry juice-making, about 1/3 of the free ellagic acid and almost one half of the ellagic acid released from ellagitannin particles were transferred into the juice. The remaining content of the free and released ellagic acid was contained in the flesh and achenes in a 2 : 1 ratio. The dried flesh produced, its amount being 1.2 % of the raw material weight, contained 73 mg of free ellagic acid, 855 mg of sanguiin H-6, and 1030 mg of the released ellagic acid in 100 g of d.m. It was shown that the dried achenes, constituting 1 % of the raw material weight, contained a similar quantity of free ellagic acid and a significantly lower amount of the released ellagic acid (710 mg/100 g d.m.). The content of sanguiin H-6 in the achenes was ca. 450 mg/100 g d.m. and was almost two times lower than in the flesh.