FOOD. Science. Technology. Quality

Food. SCIENCE. Technology. Quality

Food. Science. TECHNOLOGY. Quality

Food. Science. Technology. QUALITY

Authors

BARBARA WÓJCIK-STOPCZYŃSKA, BARBARA JAKUBOWSKA

Title

In vitro assessment of antifungal activity of some dried herbs

Abstract

In the study, there were assessed the chemical composition and the antifungal activity of some dried herbs, i.e. basil, oregano, rosemary, and thyme. The individually prepackaged dried herbs were purchased in a retail network. In the mixed and powdered samples, there were determined the contents of dry matter, total protein, total sugars, total polyphenols, and essential oils. To study the antifugal activity of herbs, twelve strains of fungi were used, i.e.: Alternaria alternata., Aspergillus flavus, A. fumigaus, A. niger, Cladosporium herbarum, Fusarium oxysporum, Penicillium cyclopium and Eurotium amstelodami, E. chevalieri, E. herbariorum, E. repens, and E. rubrum. As for the Eurotium ssp. fungi, a Dichloran Glycerol (DG-18) Agar Base was used, and for the other fungi: a Malt Extract Agar Base. The experiment included fungal cultures grown on the media with the dried herbs added (1 %) and control cultures grown on the media without the dried herbs. Sodium benzoate (0.05 %) was used as a positive control sample. On the 3rd, 6th, and 9th day of the experiment, the diameters of the analysed and the control colonies were measured and a percent rate was computed of the fungal growth inhibition caused by the dried herbs. It was found that the antifungal activity varied depending on the type of dried herbs, the fungus species, and the incubation time. On the ninth day of the experiment, the dried oregano containing the highest level of essential oil (2.40 % d.m.) and a lot of polyphenols (4.06 % d.m.) caused the growth of both the Eurotium ssp. fungi and the other fungi to be inhibited at the significantly highest mean rate (95.5 and 90.2 %, respectively). The dried basil containing the lowest amounts of oil and polyphenols and the highest amount of total proteins stimulated the growth of some part of the fungi. The dried herbs studied caused the strongest growth inhibition of the cultures of A. fumigatus, A. alternata, and E. amstelodami fungi and the weakest growth inhibition of A. niger and E. Rubrum. The growth inhibition of the fungi decreased with the time of incubation but the differences between the mean percent rates of growth inhibition on the 6th and the 9th day of the incubation period were insignificant. The dried oregano, thyme, and rosemary inhibited the growth of fungal colonies more effectively than the sodium benzoate.

Keywords

basil, oregano, rosemary, thyme, antifungal properties

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