Under the model investigations as presented herein, the content of folic acid was determined in individual stages of the technological process of producing wheat and rye bread from the flour fortified with 0.01g/100 g of folic acid. The extraction of folic acid from the products analyzed was performed according to a method described by Gujska and Kuncewicz and modified pursuant to the Polish Standard PN/EN 14131:2003; a process of tri-enzyme hydrolysis (coniugase, α-amylase, protease) was applied in the extraction. The determination of the folic acid content was carried out by a liquid chromatography method using a high performance chromatograph (La ChromElite, Hitachi) with a UV/VIS detector. It was found that the technological process of producing wheat and rye bread caused the content of the added folic acid to decrease. As for the wheat bread, the content of folic acid in the final product was by 35 % lower compared to its amount in the flour, and as for the rye bread – by as much as 62 %. The food safety aspects were taken into consideration since the technological investigations should reflect the real level of fortifying cereal products with folic acid.
folic acid, fortification, bread, technological process, HPLC