The investigations presented referred to the enzymatic hydrolysis applied to utilize non-standard artificial collagen casings and process wastes produced while producing the casings. It was stated that the collagen was practically the only protein component of the materials under analysis. There were determined conditions necessary for the proteolysis to occur; for this purpose, several selected enzyme preparations were used. Pepsin hydrolysed collagen casings to a marginal degree. Trypsin, papain, neutrase of Bacillus subtilis, alcalase of Bacillus licheniformis, and esperase of Bacillus lentus decomposed the collagen material by 62% – 66% to proteinaceous constituents of differing molecular weights, and by 34% – 38% to non-protein nitrogen components. Optimal conditions of temperature and pH for the hydrolysis of the material investigated were as follows: trypsin – 60°C, pH – 8.0; papain – 60°C, pH – 4.5; neutrase – 55°C, pH – 7.0; alcalase – 65°C, pH – 8.0; esperase – 65°C, pH – 8.0. During the enzymatic hydrolysis, more than 60% of hydroxyproline contained in the material studied were converted into compounds, which do not react with the Ehrlich’s reagent.
non-standard collagen casings, collagen wastes, enzymatic hydrolysis, pepsin, trypsin, papain, bacterial proteases